Becker & Hickl GmbH
Becker & Hickl have introduced a proprietary time-correlated single-photon counting principle that made TCSPC more than 100 times faster than the existing devices. Moreover, bh introduced a multi-dimensional TCSPC process that records the photons not only versus the time in the signal period, but also versus other parameters, such as experiment time, wavelength, or spatial coordinates. The bh devices are designed to record multi-dimensional photon distributions, time-resolved images, sequences of photon distributions, or multi-dimensional time-tag data. The bh TCSPC products are complemented by bh picosecond diode lasers, detector modules, multi-spectral detector assemblies, and experiment control modules. Based on these components Becker & Hickl supply their own confocal fluorescence lifetime laser scanning microscope and FLIM upgrade kits for laser scanning microscopes of various manufacturers. Moreover, bh is supplier of TCSPC modules for commercial time-domain optical tomography instruments. bh market activities include currently 5 workshops around the world and distribution of more than 1200 pages of TCSPC literature.
- +49 (30) 212 80 02-0
- +49 (30) 212 80 02-13
- info@becker-hickl.com
- Nunsdorfer Ring 7-9
Berlin, 12277
Germany
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Laser
BDS-SMY Series
*Pulse Width Down to 50 ps*Repetition Rate 50 MHz (20 MHz Available on Request)*Free Beam or Fiber Output*CW-Equivalent Power 0.3 mW to 0.5 mW*All Electronics Integrated*No External Driver Unit*Simple +12 V Supply*40 mm x 70 mm x 120 mm
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Fiber-Based TCSPC System
*Hybrid Fiber System With Implantable Fiber Tip*Tip Removable from Fiber System*Seperate Excitation and Detection Fibers*Extremly Low Background Fluorescence*One or Two bh ps Diode Laser (BDS-SM or BDL-SMN Series)*High-Efficiency HPM-100-40 or -50 Hybrid Detector*Optional: Multi-Wavelength Detector
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FLIM Upgrades for Olympus
FV1000
*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NIR FLIM*Optional: Multi-Wavelength FLIM*Excitation by bh ps Diode Lasers*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Simultaneous FLIM/PLIM*Detection Wavelength Range from 360 nm to 900 nm*Excitation by Ti:Sa Laser, OPO, or by bh ps Diode Lasers*Systems for Multiphoton FLIM and Confocal FLIM*Systems are Modular: More FLIM Channels can be Added
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Analysis Software for FLIM, PLIM
SPCImage
*Analysis of Single-Decay, FLIM and PLIM Data*Analysis by Iterative Convolution and Fit Procedure or by First Moment of Photon Distribution*Calculations of Decay Parameters and Lifetimes by Various Decay Models*Calculation of FRET Efficiencies*Display of Lifetime and FRET Images*Display of Lifetimes, Amplitudes, Intensities or Ratios*Export of Lifetime Data and Images
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FLIM Upgrades for Zeiss LSM
710 / 780 / 880
*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NIR FLIM*Optional: Multi-Wavelength FLIM*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Simultaneous FLIM/PLIM*Detection Wavelength Range from 360 nm to 900 nm*Excitation by Ti:Sa Laser, OPO, or by bh ps Diode Lasers*Identical Systems for Multiphoton FLIM and Confocal FLIM*Detectors can be Swapped Between NDD Port and Confocal Port*Systems are Modular: More FLIM Channels can be Added
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Instrument Software for SPC- and DPC Modules
SPCM
*Multifunctional 64 bit Data Acquisition Software*Recording of Multi-Dimensional TCSPC Results and Time-Tag Data*Control of Multi-Dimensional Recording Features*Configurable User Interface*Acquisition of Decay Curves, FCS, MCS Traces, FLIM and PLIM Images*Online Calculation and Display of FLIM, PLIM, FCS, PCH, MCS Traces and Fit of FCS Curves*Different Operation Modes Available*Measurement Control of Up to 4 Modules*Configurable for Different Instrument Configurations*Accumulation and Autosave Functions*Integrated Scanner Control*Saving, Loading and Conversion of Photon Distributions, Images and Time-Tag Data*Saving and Loading of Results and System Parameters*Integrated Control of Ti:Sa Lasers*Integrated Control of Motor Stages
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Multiphoton FLIM System
DCS-120 MP
*Multiphoton FLIM Upgrade for Existing Conventional Microscope*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Scanning by Fast Galvanometer Mirrors*Channel Seperation by Dichroic or Polarising Beamsplitters*Individually Selectable Pinholes and Filters*Excitation by Ti:Sa Laser*Two Non-Descanned Detection Channels*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: Two HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NIR FLIM*Optional: Multi-Wavelength FLIM*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Simultaneous FLIM/PLIM*Electronic Pinhole Alignment*Z-Stack FLIM Acquisition with Zeiss Axio Observer Z1*Optional: Spatial Mosaic FLIM via Motorized Sample Stage*Laser Control via SPCM Software
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Analysis Software for FCS and cross-FCS
Burst-Analyzer
*FCS Fit with User-Defined Model Functions*Identification of Single-Molecule Photon Bursts in Parameter-Tag Data*Analysis of Fluorescence Intensity and Lifetime within Photon Bursts*One- and Two-Dimensional Histograms of Burst Parameters*Filtered Histograms of Burst Parameters*Discrimination of Different Fluorescent Species or FRET States*Determination of FRET Efficiencies*Exclusion of Artifacts in Intensity Traces
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Wide-Field FLIM System
LINCam FLIM
*Easy to Use Like an Ordinal Megapixel CCD Camera*Spatial Resolution ~ 1024 x 1024 Pixels*Temporal Resolution (IRF Width) 50 ps FWHM*Single-Photon Sensitivity*Saturated Count Rate Up to 5 MHz*Excitation by bh BDS-SM Family ps Diode Laser*Works with All Commonly Used MicroscopesI*llumination via Single-Mode Fiber*Lens System for Image Transfer Into Camera
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Laser Scanning FLIM Microscopes
DCS-120 laser scanner: Compact - Flexible - Precise. As technology leader in equipment and techniques for single photon counting, Becker & Hickl offers the DCS-120 systems that are complete laser scanning microscopes for fluorescence lifetime imaging (FLIM) since 2007. The systems use bh’s multi-dimensional TCSPC FLIM technology in combination with fast laser scanning and confocal detection or multi-photon excitation.
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Detectors
Designed and manufactured by Becker & Hickl - bh offers a wide range of detector types as photomultiplier tubes (PMC), hybrid photo detectors (HPM), single-photon avalanche diodes (SPAD) as well as multi-channel- and multi-spectral detectors. The detection range covers the wavelengths from UV to the NIR and time resolutions spanning from tens of ps to hundreds of ps suitable for anti-bunching, FCS, FLIM, PLIM and other applications. bh guarantee that their TCSPC modules work with any photon counting detector and reach the fastest possible IRF width.Choose your detection now:
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Standard Lifetime Measurement System
Filter-Based Systems
*Excitation by bh ps Diode Lasers (BDS-SM or BDL-SMN Series)*Optional: fs Fiber Laser*Wavelength Selection by Filters*Detection by bh HPM-100 Hybrid Detectors*Excellent Dynamic Range*Ultra-High Time Resolution*IRF Width Down to 40 ps FWHM with HPM-100-06/07*Low Cost Detector Option: PMC-150 PMT Modules
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FLIM Upgrades for Leica
SP2 / SP5 / SP8
*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Identical Systems for Multiphoton FLIM and Confocal FLIM*Detectors can be Swapped Between RLD Port and X1 Port*Systems are Modular: More FLIM Channels can be Added
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FLIM Upgrade Kits for LSMs
As technology leader in equipment and techniques for single photon counting, Becker & Hickl offers a wide range of high-grade Fluorescence Lifetime Imaging (FLIM) systems for laser scanning microscopes since 1998. Our techniques have found broad application within the scope of Life Sciences, Clinical FLIM, Diffuse Optical Tomography (DOT), Fluorescence Correlation (FCS, FCCS), Förster Resonance Energy Transfer (FRET) and more.
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Laser
BDS-SM Series
*Pulse Width Down to 60 ps*Repetition Rate 20 MHz /50 MHz / 80 MHz / CW (Switchable)*Free Beam or Fiber Output*All Electronics Integrated*No External Driver Unit*Simple +12 V Supply*40 mm x 70 mm x 120 mm
